An Unbiased View of how HPLC works
An Unbiased View of how HPLC works
Blog Article
Since the stationary phase is polar, the cell stage is usually a nonpolar or perhaps a moderately polar solvent. The mixture of the polar stationary stage and also a nonpolar cell stage is called standard- phase chromatography
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
. HPLC separation of a mix of flavonoids with UV/Vis detection at 360 nm and, from the inset, at 260 nm. The selection of wavelength has an effect on Just about every analyte’s sign.
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
The selection from the column sort is determined by the physicochemical properties in the analytes currently being divided.
テキストはクリエイティブ・コモンズ 表示-継承ライセンスのもとで利用できます。追加の条件が適用される場合があります。詳細については利用規約を参照してください。
If you desire to to reuse any articles, in print or on the internet, from ChemistryViews.org, make sure you contact us 1st for authorization and talk more info to our authorization guidance prior to making your request.
This individual instrument involves an autosampler. An instrument wherein samples are injected manually does not include the functions revealed in The 2 left-most insets, and has a distinct sort of loop injection valve.
This variance in get more info interaction periods brings about the separation of analytes as they exit the column at unique situations.
移動相としては、カラムや装置に悪影響を与えない範囲で各種の溶媒が使用される。水や塩類の水溶液、アルコール類、アセトニトリル、ジクロロメタン、トリフルオロ酢酸などが用いられる。相溶性のある（互いに混じり合う）溶媒を混合して使用する場合が多い。
The cellular period flows with the stationary section and carries the elements in the mixture with it. Distinctive elements travel at different prices. So the parts separated and located in different region in chromatography to separate, identify and quantify.
If the solution is diluted the area of the height will be considerably less, though the detention time will likely be exact same. Consequently it is feasible to detect a compound present even in an extremely small amount.
The sample injector introduces the sample into the HPLC system. Specific and exact sample injection is important for obtaining trustworthy results.
A quantitative HPLC Evaluation is commonly less complicated than the usual quantitative GC Examination since a hard and fast quantity sample loop offers a more precise and exact injection.